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Previous data showed that acute metformin treatment stimulated the release of glucagon-like peptide 1 in a cholinergic activity-dependent manner in rats ( Funding This work was supported by the Brazilian Federal Foundation, the Conselho Nacional de Desenvolvimento Cientfico e Tecnolgico (CNPq), the Coordenao de Aperfeioamento de Pessoal de Nvel Superior (CAPES), and the Paran Science Foundation (Fundao Araucria) and Science without Borders Program (Programa Ciencias Sem Fronteiras)

In cytokine-stimulated astrocytes, arginine deprivation activates the amino acid-sensing kinase GCN2, leading to phosphorylation of eukaryotic initiation factor 2 (eIF2) and suppression of iNOS protein synthesis, thereby attenuating NO production

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